Table of Contents
- 1 What happens if the centrifuge is not balanced?
- 2 Why is it important to balance the centrifuge before using it?
- 3 When operating a centrifuge What happens if the centrifuge is not properly balanced quizlet?
- 4 Why is it important to not stop the centrifuge abruptly?
- 5 How does speed affect centrifugation?
- 6 How does centrifugal force help centrifugation?
- 7 What analytes increase and decrease with delayed centrifugation?
- 8 What should you do in the event of a centrifuge failure when the centrifuge begins to shake or walk?
- 9 Why to use a centrifuge?
- 10 What does a centrifuge do exactly?
What happens if the centrifuge is not balanced?
What do I have to do if an imbalance error occurs? If the centrifuge begins to shake or wobble, it is off-balance and you should stop it immediately. A little vibration is normal, but excessive amounts can mean danger.
Why is it important to balance the centrifuge before using it?
Balance the centrifuge Running an unbalanced centrifuge may cause significant damage, and injure the operator and other laboratory personnel. The total mass of each tube should be as close as possible- this becomes increasingly important at very high rotor speeds.
What factors affect centrifugation?
Which factors have an influence on centrifugation :
- Density of both samples and solution.
- Temperature/viscosity.
- Distance of particles displacement.
- Rotation speed.
When operating a centrifuge What happens if the centrifuge is not properly balanced quizlet?
2 what will happen if the centrifuge is not properly balanced? if a centrifuge is not properly balanced, it can injure you and ruin the centrifuge as well. they operate at high speeds and unbalanced can result in the sample container breakage, which can be potentially harmful depending on the sample type.
Why is it important to not stop the centrifuge abruptly?
This can result in injury AND it will stir up the solution you were attempting to separate by centrifugation. Wait for the rotor to come to a complete stop before attempting to remove your test tubes. After about 30 seconds of centrifugation most precipitates will have settled.
How do you properly balance a centrifuge if an odd number of samples must be centrifuged?
If you have an even number of sample tubes, place them directly across from each other in the rotor. For an uneven number of samples, add another test tube to the rotor that’s filled with liquid of the same volume and density to keep your load balanced.
How does speed affect centrifugation?
Doubling the speed of rotation increases the centrifugal force by a factor of four. The centrifugal force also increases with the distance from the axis of rotation. These two parameters are of considerable significance when selecting the appropriate centrifuge.
How does centrifugal force help centrifugation?
Centrifugation, the name given to separation applications which involve spinning around an axis to produce a centrifugal force, is a way to increase the magnitude of the gravitational field. The particles in suspension experience a radial centrifugal force moving them away from the axis of rotation.
Why do you think it is important to have a balanced centrifuge quizlet?
Explain why it is important that a centrifuge carry a balanced load. A centrifuge must carry a balanced load; otherwise, the rotor of the centrifuge may spin out of center, which can damage the centrifuge and cause samples to break.
What analytes increase and decrease with delayed centrifugation?
Vitamin D, zinc, ferritin and creatinine levels increased with higher temperatures and delays in centrifugation. Creatinine was particularly affected even when exposed to room temperature for 24 h.
What should you do in the event of a centrifuge failure when the centrifuge begins to shake or walk?
If the centrifuge starts making a loud noise or starts shaking, the load is most likely out of balance. Stop the run, allow the centrifuge to come to a stop and reload your samples after balancing.
What happens if a centrifuge is unbalanced?
Aerosols can be created when a centrifuge tube or sample tube breaks inside of the rotor. Additionally, an unbalanced rotor can create instability within the equipment, which can be greatly multiplied by the operating speed of the centrifuge and can cause user injury or laboratory damage.
Why to use a centrifuge?
Types and Uses of Centrifuges Separating Molecules and Isotopes: Extremely high-speed centrifuges and ultracentrifuges spin at such high rates that they can be used to separate molecules of different masses or even isotopes of atoms. High-Gravity Simulation: Large centrifuges may be used to simulate high-gravity. Industrial Centrifuges.
What does a centrifuge do exactly?
A small centrifuge used for the precipitation of proteins and other biological substances. A centrifuge is a piece of equipment that puts an object in rotation around a fixed axis (spins it in a circle), applying a force perpendicular to the axis of spin (outward) that can be very strong.
How is a centrifuge balanced?
When there is an even number of samples, the centrifuge can be balanced by dividing the total number of tubes in half and placing the two sets into the rotor directly opposite each other to create an equal number of tubes on each side.