Table of Contents
- 1 What is the function of a mordant in staining?
- 2 What is used as the mordant in the Gram staining technique quizlet?
- 3 What is a mordant in microbiology?
- 4 What is used as a mordant in this Endospore staining method?
- 5 What is the mordant in the Endospore stain?
- 6 What is the mordant used in the Gram stain procedure quizlet?
- 7 What are the steps in a Gram stain?
- 8 What is a Gram staining procedure?
What is the function of a mordant in staining?
A mordant or dye fixative is a substance used to set (i.e. bind) dyes on fabrics by forming a coordination complex with the dye, which then attaches to the fabric (or tissue). It may be used for dyeing fabrics or for intensifying stains in cell or tissue preparations.
What is a mordant in Gram staining?
The mordant is Gram’s Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane.
What is used as the mordant in the Gram staining technique quizlet?
The mordant used is Iodine. It is added to chemically change the shape of the dye molecule and therefore trap it in the cell wall.
Why is a mordant used in the Gram stain in the flagella stain?
Principle: Because bacterial flagella are very thin and fragile a special stain (flagella stain) is prepared that contains a mordant. This mordant allows piling of the stain on the flagella, increasing the thickness until they become visible. Various arrangements of flagella are seen on different cells.
What is a mordant in microbiology?
A mordant is a substance used to set or stabilize stains or dyes; in this case, Gram’s iodine acts like a trapping agent that complexes with the crystal violet, making the crystal violet–iodine complex clump and stay contained in thick layers of peptidoglycan in the cell walls.
What is a mordant in histopathology?
A mordant is a chemical that serves as a link between the dye and the substrate. The result is an insoluble compound that helps adhere the dye to the cells.
What is used as a mordant in this Endospore staining method?
What serves as the mordant in the endospore staining method? Safranin, final staining step. Binds with phospholipid membrane of both cells. Positive are purple and negative are pink/red.
Why is a mordant used in the Gram stain quizlet?
What is the mordant in the Endospore stain?
Answer and Explanation: Reagents used in the endospore staining are. Malachite green (5\%) is a primary stain. It stains the endospores of the bacterial cells. Heat is a mordant, which is useful for fix the stain or dye on a microorganism.
What is the purpose of mordant in Gram staining quizlet?
What is the mordant used in the Gram stain procedure quizlet?
Discuss the purpose of a mordant and list the mordant used in the Gram Stain? The mordant used is Iodine.
Which is used as mordant?
Salts of chromium, aluminum, copper, iron, tin, and cobalt are commonly used as mordants. Since the mordant affects the electron distribution and density within the dye, the color of the dyed fabric tends to change.
What are the steps in a Gram stain?
There are four basic steps of the Gram stain: Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture. The addition of iodide , which binds to crystal violet and traps it in the cell Rapid decolorization with ethanol or acetone Counterstaining with safranin.
What is the Gram stain method Quizlet?
The Gram stain is a differential method of staining used to assign bacteria to one of two groups (gram-positive and gram-negative) based on the properties of their cell walls. It is also known as Gram staining or Gram’s method. The procedure is named for the person who developed the technique, Danish bacteriologist Hans Christian Gram.
What is a Gram staining procedure?
Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. A counterstain, such as the weakly water soluble safranin, is added to the sample, staining it red.
What is Gram stain in bacteria?
Gram stain. A Gram stain is a test used to identify bacteria. It is one of the most common ways to quickly diagnose bacterial infection in the body.