Table of Contents
- 1 How does temperature affect DNA extraction?
- 2 Would you expect the same method of DNA extraction we used for the fruit to be the same for human DNA?
- 3 Why must DNA extraction occur at ice cold temperatures?
- 4 What is DNA extraction?
- 5 How is DNA extraction useful to scientists?
- 6 Is it possible to extract DNA from a banana?
- 7 How do you separate DNA from a banana fruit?
How does temperature affect DNA extraction?
Temperature has a significant effect on the amount of DNA that can be extracted: the lower the temperature, the greater the yield of DNA. Hence, whenever possible, specimens should be kept at cold temperatures, preferably frozen.
Why is extracting DNA from a plant cell different from extracting DNA from an animal cell?
The key difference between plant and animal DNA extraction is that plant DNA extraction requires the grinding of plant tissue in dry ice or liquid nitrogen in order to break the cell wall while animal DNA extraction does not require this step since they animal cells do not have a cell wall.
Would you expect the same method of DNA extraction we used for the fruit to be the same for human DNA?
2. In order to study our genes, scientists must extract the DNA from human tissue. Would you expect the method of DNA extraction we used for the strawberry to be the same for human DNA? Yes, in a multicellular organism (such as a human or a strawberry), all cells contain the same DNA.
Do you think it would be easier or harder to extract DNA from animal cells than from plant cells?
My hypothesis is that it is more difficult to extract DNA from plant cells due to their stiff cell wall that is not found in animal cells. The amount of DNA extracted from plant cells should increase by adding the enzyme cellulase, which breaks down plant cell walls.
Why must DNA extraction occur at ice cold temperatures?
Why is cold important? Using ice-cold water and ice-cold alcohol will increase your yield of DNA. The cold water protects the DNA by slowing down enzymes that can break it apart.
Why must DNA extraction take place at cold temperatures?
They are there to protect the cell from invasion by viruses. Once the nuclear membrane is destroyed by the soap, the DNA is now susceptible to the DNases and will quickly be degraded. However, these enzymes are temperature sensitive and cooling the solution slows down the process of degradation.
What is DNA extraction?
DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components. Manual methods as well as commercially available kits are used for DNA extraction.
How is DNA extracted from fruit?
Extracting DNA from fruit
- Peel the skin from half a kiwi fruit and mash it up.
- Mix a teaspoon of salt and small volume of washing up liquid into the fruit.
- Gently heat this mixture at about 60°C for five minutes.
- Filter the mixture and retain only the filtrate (the filtered liquid)
How is DNA extraction useful to scientists?
The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity.
How do you extract DNA from a banana?
- Step 1: Chop up the banana. Place the banana onto a plate.
- Step 2: Put the banana into a bag. Place the banana pieces into a sealable plastic bag.
- Squash the banana.
- Step 4: Add salt to warm water.
- Step 5: Add washing up liquid.
- Step 6: Pour into the bag.
- Step 7: Sieve.
- Step 8: Pour the drained liquid into a glass.
Is it possible to extract DNA from a banana?
Well, not entirely. Because DNA is in every cell, there is a lot of it in an organism. If you took all of the DNA out of some middle-sized organism (or part of an organism, like a piece of fruit), you could see and even touch DNA. We will use common household products to break apart the cells in a banana and extract out the DNA.
How do bananas pass information from one generation to another?
All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. Within every living organism, most cells contain a complete set of DNA instructions. The information in DNA tells our bodies how to develop, grow, and work.
DNA Extraction process DNA is an incredibly small molecule, but in large quantities, it can be seen. In this activity, you will extract DNA from banana, strawberry and kiwi fruits. We are going to use fruits because they are soft and easy to pulverize. There are three basic steps in DNA extraction.
How do you separate DNA from a banana fruit?
During this experiment, a banana fruit was crushed, and proteins and cell membranes of the fruit deteriorated by adding a soapy solution to them. The cells then were then filtered and mixed with an ethanol solution to separate the DNA from the rest of the fruit sample.