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How are Okazaki fragments on the lagging strand joined into one continuous strand quizlet?
Small fragments of DNA produced on the lagging strand during DNA replication, joined later by DNA ligase to form a complete strand.
How Okazaki fragments are formed on lagging strand?
Okazaki fragments are formed on lagging strands, initiated by the creation of a new RNA primer by the primosome. Okazaki fragments are formed on the lagging strand for the synthesis of DNA in a 5′ to 3′ direction towards the replication fork. The ligase enzyme joins the Okazaki fragments together, making one strand.
How do Okazaki fragments form?
Okazaki fragments are initiated by creation of a new RNA primer by the primosome. To restart DNA synthesis, the DNA clamp loader releases the lagging strand from the sliding clamp, and then reattaches the clamp at the new RNA primer. Then DNA polymerase III can synthesize the segment of DNA.
What strand do Okazaki fragments form?
Okazaki fragments are the short DNA fragments on the lagging strand formed during DNA replication. Since the lagging strands run in the 3′ to 5′ direction, the DNA synthesis on the lagging strand is discontinuous. It forms Okazaki fragments on the lagging strand that are ligated later by DNA ligase.
Why are Okazaki fragments joined together?
Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.
What events must take place to make the lagging strand continuous?
Lagging strand synthesis requires (1) protection of the single-stranded DNA (ssDNA) as it is extruded by the helicase, (2) multiple initiation events by a primase, (3) removal of the RNA primer, (4) ligation of the Okazaki fragments, and (5) transfer of the polymerase from the completed fragment to a new primer.
Is the lagging strand continuous?
This enzyme can work only in the 5′ to 3′ direction, so it replicates the leading strand continuously. Lagging-strand replication is discontinuous, with short Okazaki fragments being formed and later linked together.
Why is the lagging strand discontinuous?
On the upper lagging strand, synthesis is discontinuous, since new RNA primers must be added as opening of the replication fork continues to expose new template. This produces a series of disconnected Okazaki fragments.
Why lagging strand synthesis is discontinuous?
Why are Okazaki fragments formed on lagging strand only Brainly?
Answer: Okazaki fragments are formed on the lagging strand so that DNA can be synthesized in the essential 5′ to 3′ manner on the lagging strand.
What are 4 events that must occur in the lagging strand?
Why is the lagging strand replicated in fragments?
Lagging strand is synthesised in fragments. Nucleotides cannot be added to the phosphate (5′) end because DNA polymerase can only add DNA nucleotides in a 5′ to 3′ direction. The lagging strand is therefore synthesised in fragments.